2Yrd. Doç. Dr., Harran Üniversitesi Tıp Fakültesi, Biyokimya AD, Şanlıurfa-Türkiye
3Yrd. Doç. Dr., Kafkas Üniversitesi Tıp Fakültesi, Göz Hastalıkları AD, Kars-Türkiye
4Prof. Dr.,Harran Üniversitesi Tıp Fakültesi, Biyokimya AD, Şanlıurfa-Türkiye Purpose: To investigate the total oxidant status (TOS), total antioxidant capacity (TAC) and oxidative stress index (OSI) of the aqueous humor and serum in patients with mature and immature senile cataract.
Materials and Methods: Cataract surgery patients (n=34) were enrolled in the study. We included 17 patients with mature cataracts (Group 1) and 17 patients with immature cataracts (Group 2). Samples of the aqueous humor and serum were taken from all patients and measured spectrophotometrically. Serum and aqueous TOS, TAC and OSI levels were compared in the two groups. The Kolmogorov-Smirnov test and Mann-Whitney U test were used for statistical analyses.
Results: The mean TOS level in Group 1 and 2 patients was 508.7 ± 45.9 and 245.6 ± 15.8, respectively, which is a statistically significant difference (p=0.0001). The mean TAC level in Group 1 and 2 was 2.28 ± 0.6 and 3.83 ± 2.1, respectively. This difference was statistically significant (p=0.005). The mean OSI in Group 1 and 2 was 13.9 ± 4.1 and 10.4 ± 1.4, respectively, with the mean OSI level statistically higher in Group 1 (p=0.005). The differences in mean TOS, TAC and OSI levels in the serum were not statistically significant.
Conclusion: The aqueous humor of mature cataract patients is characterised by increased oxidative stress and decreased antioxidant capacity. The increased oxidative stress may play a significant role in the pathogenesis and maturation of senile cataracts. Keywords : Aqueous humor, cataract, serum, oxidative stress
