Material and methods: Experiments were performed in-vitro with total 15 glass tubes of each filled with 1.5 ml of fl uid media were prepared. The tubes were then divided equally into 3 groups to culture the bacteria staphylococcus (S.) aureus, pseudomonas (P.) aeruginosa and fungus candida (C.) albicans. In a set of 5 tubes, colony of each microorganism was thus produced. In each group, Tube (numbered 0) was established as a control. The remaining Tubes 1-4 were respectively added with 1 to 4 drops of CoQ10 containing eye drops. The tubes were then evaluated at 0, 1, 2, 4, 5 and 24 hours. The proliferation of microorganism in a liquid medium resulted in turbidity from which microbial concentration was estimated using McFarland barium sulphate turbidity standard. Measurements were performed on the spectrophotometer as McFarland standard and interpreted through the changes in reproduction. For each group, the estimates of the average numbers of the corresponding microorganisms subjected to the four different strengths of CoQ10 were recorded and statistically compared to those in the control group.

Results: In all the groups, the concentrations of the microorganisms (S. aureus, P. aeruginosa and C. albicans) increased with time. However, exposure to CoQ10 containing eye drops did not signifi cantly alter the microorganismal growth as the measurements from the CoQ10-instilled tubes remained comparable to those of the controls.

Conclusion: CoQ10 under in-vitro conditions did not induce proliferation of S. aureus, P. aeruginosa and C. albicans. Keywords : ocular microbiata, coenzyme Q, ocular surface